• VALIDATED SPECTROPHOTOMETRIC METHOD FOR SIMULTANEOUS ESTIMATION OF AMLODIPINE BESILATE, OLMESARTAN MEDOXOMIL AND HYDROCHLOROTHIAZIDE IN THEIR COMBINED DOSAGE FORMS

Abstract

A method was developed for the simultaneous estimation of amlodipine besilate, olmesartan medoxomil and hydrochlorothiazide in tablet dosage form by using methanol as solvent. Three wavelength selection method were apply, in which three analytical wavelengths 360 nm, 253 nm, 270 nm were selected for amlodipine besilate ,olmesartan medoxomil  and hydrochlorothiazide respectively. Shimadzu 1800 capable of multi component analysis was used for quantification. In this method estimation of amlodipine besylate was carried out by plot a calibration curve absorbance Vs concentration at 360 nm. Where as olmesartan medoxomil and hydrochlorothiazide were estimated by simultaneous equation method.  The method was found to be liner at concentration range 5-19 ppm 4-32 ppm and 2-23 ppm for amlodipine besylate ,olmesartan medocxomil and hydrochlorothiazide with r² 0.998, 0.999 and o.996 respectively. The method was found to be accurate with % recovery 99.86 % for amlodipine besylate, 100.30 % for olmesartan medoxomil and 99 % for hydrochlorothiazide. precision was found 1.48, 1.16 and 1.07 intra day and 0.99, 2.04  and 1.23 inter day for amlodipine besylate ,olmesartan medoxomil and hydrochlorothiazide respectively. LOD was found 0.21, 0.22 and 0.07 and LOQ was found 0.66, 0.60 and 0.30 respectively.