• ACUTE AND SUB ACUTE TOXICITY STUDIES OF A FEMALE INFERTILITY SIDDHA DRUG - KARPA CHOORANAM ON MICE

Abstract

Female Infertility is a pan global public health issue leading to physical, emotional and social stigmatic conditions. Ovulatory factors play a major role in female infertility. The Siddha herbal formulation Karpa Chooranam (KC) has been employed for the treatment of Female Infertility (FI). The safety profile of KC was evaluated in mice using OECD guidelines. In Acute oral toxicity study, a single dose of KC was administered and observed for 14 days. The results of acute toxicity study of Karpa Chooranam revealed no mortality, abnormal signs and behavioral changes in mice at the dose of 2000mg/ kg body weight. Sub-acute toxicity studies were carried in four different groups in which KC was administrated orally to mice once daily for 28 days in various doses ranging from 104, 520 and 1040 mg/kg body weight respectively. Detailed hematological, biochemical, necropsy and histopathological evaluation of organs were performed for all animals. KC was well tolerated and no toxic manifestations were seen in any of the animals. Histopathological analysis revealed that Spleen, Testes, Pancreas, Lung, Intestine, Stomach, Liver, Brain, Heart, Ovary, Uterus and Kidney tissues of treated groups did not show any signs of toxicity. No toxic effect was observed in both acute and sub-acute toxicity studies of Karpa Chooranam.